1.0FluoroFinderhttps://fluorofinder.comIleniahttps://fluorofinder.com/author/ilenia/rich600338<blockquote class="wp-embedded-content" data-secret="vvB1IS7x4A"><a href="https://fluorofinder.com/newsletter-background-fluorescence/">Newsletter: Background Fluorescence</a></blockquote><iframe sandbox="allow-scripts" security="restricted" src="https://fluorofinder.com/newsletter-background-fluorescence/embed/#?secret=vvB1IS7x4A" width="600" height="338" title="“Newsletter: Background Fluorescence” — FluoroFinder" data-secret="vvB1IS7x4A" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" class="wp-embedded-content"></iframe><script type="text/javascript"> /* <![CDATA[ */ /*! This file is auto-generated */ !function(d,l){"use strict";l.querySelector&&d.addEventListener&&"undefined"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[^a-zA-Z0-9]/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret="'+t.secret+'"]'),o=l.querySelectorAll('blockquote[data-secret="'+t.secret+'"]'),c=new RegExp("^https?:$","i"),i=0;i<o.length;i++)o[i].style.display="none";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute("style"),"height"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener("message",d.wp.receiveEmbedMessage,!1),l.addEventListener("DOMContentLoaded",function(){for(var e,t,s=l.querySelectorAll("iframe.wp-embedded-content"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute("data-secret"))||(t=Math.random().toString(36).substring(2,12),e.src+="#?secret="+t,e.setAttribute("data-secret",t)),e.contentWindow.postMessage({message:"ready",secret:t},"*")},!1)))}(window,document); /* ]]> */ </script> https://fluorofinder.com/wp-content/uploads/2019/09/Background-Fluor-LB.png345227Background fluorescence includes any signal detected beyond what is generated by the fluorochromes being measured.  The three major sources of background fluorescence include autofluorescence, spectral overlap, and undesirable antibody binding. Because false signals can significantly skew the resulting data and inhibit the ability to detect weakly positive cell populations, researchers must reduce background fluorescence wherever […]